Recombinant COVID-19 Mouse monoclonal antibody (mab) therapy
Background
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
| Name | COVID-19 Mouse Monoclonal Antibody 40021-T62 Recombinant HCoV-HKU1 Spike Protein | Application | Invitro research and manufacture use;
membrane assay, ELISA assay, |
| Formulation | 0.2 μm filtered solution in PBS | Buffer: | 1X PBS buffer |
| Storage: | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative- Free. Avoid repeated freeze-thaw cycles. | Concentration | WB: 1:1000-1:5000 ELISA: 1:5000-1:10000 |
| Expiration Date: | 30 days refrigerated at -2-8ºC
and twelve months frozen at -20 ºC
or below from production date.
To be evaluated independently | Feature | High Sensitivity and Specificity |
Specification Data
| Description: | Recombinant HCoV-HKU1 Spike Protein |
| Clone: | SARS-CoV-2 (2019-nCoV) Spike RBD Protein | |
| Source: | Rabbit IgG | |
| Immunogen: | HCoV-HKU1 (isolate N1) Spike S1 Protein HCoV-HKU1 (isolate N5) Spike S1 Protein HCoV-NL63 Spike S1 Protein | |
| Formulation: | 0.22 μm filtered solution in Histidine and Arginine buffer containing 120mM NaCl, 0.02% Tween 80 | |
| Purification: | DEAE chromatography | |
| Concentration: | 1.0 mg/mL (OD280 nm, E0.1% = 1.40) |
| Affinity Constant: | Not determined | |
| Buffer: | 0.015M potassium phosphate buffer, 0.85% NaCl, 0.05% NaN3 pH=7.2 |
| Pairing Assays: | N/A | |
| Purity: | >95% by HPLC and SDS-PAGE |
| Application: | WB, ELISA, IHC-P, FCM, ICC/IF, IP IHC, FCM, IF, IP et al. applications haven't been validated. (Antibody's applications haven't been validated with corresponding virus positive samples. Optimal concentrations/dilutions should be determined by the end user.) |
Information on toxicological effects
Acute toxicity:No data available.
Skin corrosion/irritation: No data available
Serious eye damage/eye irritation: No data available.
Sensitization : No sensitizing effects known.
Specific target organ toxicity - repeated exposure: No data available.
Aspiration hazard: No data available.
Additional Information: Not available.
FAQ
1. Q:Are you a factory or trading company?
A: We are a factory, we have rich experience for manufacturing Medical Device more than 10 years.
2.Q:Where is your factory located? How can i visit there?
A: Our factory is located in Jiaxing city, Zhejiang Province, China.
It is about more than 1 hours from Shanghai.
2. 3.Q:Can you make samples?
A: Yes, we can make samples for you.
4.Q:How does your factory do as quality control?
A: Quality is the lift of our factory. We have inspector in different post, and there are three inspectors before packing to get best quality products.
5.Q: How is your post market service ?
A: If any damaged rings in the course of carriage, with the checking it's our mistake, we will take full responsibility to compensate the customers.
6.Q:Can you do OEM for us?
A: Certainly. We also can build the moulding for our new customer.
7.Q:Does the price include freight?
A:No, please choose the express service provider or negotiate with us.
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Recombinant COVID-19 Mouse Monoclonal Antibody (Mab) Therapy Images
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